Determination of Ochratoxin A in the rainbow trout (Oncorhynchus mykiss) feed in Chaharmahal Va Bakhtiary province by ELISA assay

Abstract

Ochratoxins are considered as the significant mycotoxins found in animal feeds. Amongst, Ochratoxin A has high pathological consequences on the humans and animals. The aim of present study was to determine the amount of Ochratoxin A in rainbow trout feed produced in Chaharmahal Va Bakhtiary province. For this, four major producers of trout feed were chosen and four different sizes of feed together with one wheat flour sample were obtained from each factory. The samples were transferred to Food Analysis Lab of Shahre-Kord Islamic Azad University. The samples were obtained in three replicates and a total of 60 samples were analyzed for the presence of Ochratoxin A. The analysis was performed by ELISA assay. Results revealed that the quantity of Ochratoxin A in all feed samples were lower than determined contamination level established by Iranian National Standard and EU commission (5µg/kg). However, the contamination levels in all wheat flour samples were higher than defined standard. The amount of Ochratoxin A in samples obtained from various producers was not statistically significant (p

Keywords

• rainbow trout
• Ochratoxin A
• ELISA
• Fish feed