Structural characterization and macrophage immunomodulatory activity of a polysaccharide isolated from Gracilaria lemaneiformis

Yinglang Ren; Guiqing Zheng; Lijun You; Lingrong Wen; Chao Li; Xiong Fu; Lin Zhou

Journal of Functional Foods (Jun 2017)

Structural characterization and macrophage immunomodulatory activity of a polysaccharide isolated from Gracilaria lemaneiformis

Yinglang Ren,
Guiqing Zheng,
Lijun You,
Lingrong Wen,
Chao Li,
Xiong Fu,
Lin Zhou

Affiliations

Yinglang Ren: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province 510640, China
Guiqing Zheng: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province 510640, China
Lijun You: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province 510640, China; Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USA; Corresponding authors at: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Lingrong Wen: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province 510640, China
Chao Li: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Corresponding authors at: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Xiong Fu: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province 510640, China
Lin Zhou: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China

Journal volume & issue: Vol. 33
pp. 286 – 296

Abstract

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Polysaccharides from Gracilaria lemaneiformis possess various beneficial bioactive functions, but the immunomodulatory activity remains incompletely known. In the present study, a new low molecular-weight sulfated polysaccharide named as GLP-2 was isolated from G. lemaneiformis. The chemical composition and structural characteristics of GLP-2 were determined by high-performance gel permeation chromatography (HPGPC), gas chromatography (GC), infrared (IR) spectrum, methylation and nuclear magnetic resonance (NMR) analyses. In addition, the immunomodulatory activity and underlying mechanism of GLP-2 were examined using murine macrophage RAW264.7 cells. The results indicated GLP-2 could significantly improve the proliferation and pinocytic capability of RAW264.7 cells, and promote the production of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by activating iNOS, IL-6 and TNF-α gene expressions. These results suggested that GLP-2 could be developed as a potent immunomodulatory agent for use in functional foods and dietary supplements.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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