Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology (May 2024)

Microbial Diversity of Aquatic Environment as Source of Enzymes

  • Tamara SÎRBU,
  • Svetlana BURȚEVA,
  • Nina BOGDAN-GOLUBI,
  • Maxim BÎRSA,
  • Valerina SLANINA,
  • Olga ȚURCAN,
  • Cristina MOLDOVAN

DOI
https://doi.org/10.15835/buasvmcn-fst:2023.0023
Journal volume & issue
Vol. 81, no. 1

Abstract

Read online

This research consisted of studying the ability of new strains of microorganisms isolated from water of the "La izvor" lake system to synthesize enzymes: amylase, catalase, cellulase, lipase and selecting those with significant enzymatic potential. Express tests were performed to determine the enzymatic capacity: amylase, catalase, cellulase and lipase of 25 strains of actinobacteria, 25 strains of bacteria, 8 strains of microalgae and cyanobacteria, 35 strains of fungi. When determining the enzymatic capacity, specific indicators were used for each enzyme: amylase - Lugol's solution, catalase - H2O2 (3%), cellulase – carboxymethyl cellulose and Congo red, lipase – Tween 80. As a result of this study, 3 strains of actinobacteria, 9 strains of bacteria, 4 strains of fungi, and 5 strains of microalgae and cyanobacteria with significant enzymatic potential as prospects for biotechnology were selected.

Keywords