Microbial Diversity of Aquatic Environment as Source of Enzymes
Abstract
This research consisted of studying the ability of new strains of microorganisms isolated from water of the "La izvor" lake system to synthesize enzymes: amylase, catalase, cellulase, lipase and selecting those with significant enzymatic potential. Express tests were performed to determine the enzymatic capacity: amylase, catalase, cellulase and lipase of 25 strains of actinobacteria, 25 strains of bacteria, 8 strains of microalgae and cyanobacteria, 35 strains of fungi. When determining the enzymatic capacity, specific indicators were used for each enzyme: amylase - Lugol's solution, catalase - H2O2 (3%), cellulase – carboxymethyl cellulose and Congo red, lipase – Tween 80. As a result of this study, 3 strains of actinobacteria, 9 strains of bacteria, 4 strains of fungi, and 5 strains of microalgae and cyanobacteria with significant enzymatic potential as prospects for biotechnology were selected.
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