Folic acid promotes myoblast migration through Folr1 interacting with RhoA and improves skeletal muscle regeneration

Shuang Li; Xin Wen; Huaixin Teng; Yunqin Yan; Huili Tong; Bingchen Liu; Shufeng Li; Shuai Shi; Jingyan Su

Journal of Functional Foods (Jan 2024)

Folic acid promotes myoblast migration through Folr1 interacting with RhoA and improves skeletal muscle regeneration

Shuang Li,
Xin Wen,
Huaixin Teng,
Yunqin Yan,
Huili Tong,
Bingchen Liu,
Shufeng Li,
Shuai Shi,
Jingyan Su

Affiliations

Shuang Li: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China
Xin Wen: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China
Huaixin Teng: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China
Yunqin Yan: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China
Huili Tong: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China; Corresponding authors at: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China (H. Tong). Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China (B. Liu).
Bingchen Liu: Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Corresponding authors at: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China (H. Tong). Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China (B. Liu).
Shufeng Li: Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China
Shuai Shi: Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China
Jingyan Su: Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China

Journal volume & issue: Vol. 112
p. 105965

Abstract

Read online

Folic acid (FA) plays multiple roles in many biological processes. Myoblast intakes FA through endocytosis by folate receptor 1 (Folr1), which has vital role in regulating muscle development. However, the mechanism remains unclear. In this study, FA supplement experiment showed that FA promoted C2C12 cell differentiation through Folr1. Further exploration found that FA could promote C2C12 cell migration through Folr1 interacting with RhoA. Then stimulated RhoA/ROCK/LIMK/cofilin signaling pathway, and affected the distribution of microfilaments to improve C2C12 cell migration. Meanwhile, mice experiments also proved that FA could activate the expression of RhoA/ROCK/LIMK/cofilin signaling pathway in vivo and accelerate the process of skeletal muscle regeneration in mice. This discovery is helpful to further understand the molecular mechanism of FA regulating muscle development and provide new ideas for the treatment of muscle diseases.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

About the journal

Abstract

Keywords