Inhibitory effects of Pleurotus tuber-regium mycelia and bioactive constituents on LPS-treated RAW 264.7 cells

Yen-Wenn Liu; Hui-Ching Mei; Yu-Wen Su; Hsiu-Tzu Fan; Chien-Chih Chen; Ying-Chieh Tsai

Journal of Functional Foods (Mar 2014)

Inhibitory effects of Pleurotus tuber-regium mycelia and bioactive constituents on LPS-treated RAW 264.7 cells

Yen-Wenn Liu,
Hui-Ching Mei,
Yu-Wen Su,
Hsiu-Tzu Fan,
Chien-Chih Chen,
Ying-Chieh Tsai

Affiliations

Yen-Wenn Liu: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 122, Sec. 2, Linong Street, Taipei 11221, Taiwan, ROC
Hui-Ching Mei: Department of Science Education, National Taipei University of Education, 134, Sec. 2, Heping E. Rd., Da-an District, Taipei 10671, Taiwan, ROC
Yu-Wen Su: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 122, Sec. 2, Linong Street, Taipei 11221, Taiwan, ROC
Hsiu-Tzu Fan: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 122, Sec. 2, Linong Street, Taipei 11221, Taiwan, ROC
Chien-Chih Chen: Department of Biotechnology, Hungkuang University, 34 Chung-Chie Road, Sha Lu, Taichung 44302, Taiwan, ROC; Corresponding authors. Tel.: +886 4 2631 8652; fax: +886 2 2826 4843 (C.-C. Chen). Tel.: +886 2 2826 7125; fax: +886 2 2826 4843 (Y.-C. Tsai).
Ying-Chieh Tsai: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 122, Sec. 2, Linong Street, Taipei 11221, Taiwan, ROC; Corresponding authors. Tel.: +886 4 2631 8652; fax: +886 2 2826 4843 (C.-C. Chen). Tel.: +886 2 2826 7125; fax: +886 2 2826 4843 (Y.-C. Tsai).

Journal volume & issue: Vol. 7
pp. 662 – 670

Abstract

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Pleurotus tuber-regium (PTR) is an edible and medicinal mushroom found mainly in tropical and subtropical countries. In this study, we investigated the anti-inflammatory effects of PTR mycelia on RAW 264.7 macrophages. Samples of PTR mycelia were prepared by submerged fermentation. An ethanolic extract of PTR mycelia (PTRA) inhibited the production of nitric oxide (NO), TNF-α, and IL-6 in LPS-treated RAW 264.7 cells. Two compounds were purified and identified as cerevisterol (CE) and ergosta-4,6,8(14),22-tetraen-3-one (EG). Both CE and EG inhibited the production of NO, TNF-α, and PGE2 in LPS-treated RAW 264.7 cells. The expression of the iNOS and COX2 proteins was inhibited by CE and EG in a dose-dependent manner. In addition, CE and EG repressed the expression of iNOS, COX2, TNF-α, and SOCS3 mRNAs. This study revealed that an ethanolic extract of PTR mycelia exhibited anti-inflammatory activity and that CE and EG might contribute to this activity.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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