Lactobacillus plantarum GMNL-662 and Lactobacillus plantarum 299v prevent osteoporosis in mice with colitis by down-regulating Akkermansia in the gut microbiome

Abstract

The effects of probiotics Lactobacillus plantarum GMNL-662 (Lp662) and Lactobacillus plantarum 299v (Lp299v) treatment on severe inflammatory bowel disease induced by dextran sodium sulfate was assessed. Male C57BL/6J mice (6 weeks old) were administered 0.9% NaCl, Lp662, or Lp299v by gavage during the entire experiment. After 3 weeks, gut inflammation was associated with bone loss, and compared to the control group, the weight loss caused by colitis in the Lp662 and Lp299v groups decreased (P < 0.001). Pathological sections of the colon showed that probiotics reduced tissue injury, and serum and tissue inflammatory factors decreased significantly (P < 0.05) compared to the control group. Three-dimensional reconstruction images showed that the bone mass in the Lp662 and Lp299v groups was significantly higher than that in the control group. This study highlights the negative impact of chronic gut inflammation on the bone and the therapeutic potential of probiotics as an anti-inflammatory treatment.

Keywords

• Lactobacillus plantarum GMNL-662
• Lactobacillus plantarum 299v
• Probiotics
• Colitis
• Inflammatory bowel diseases
• Osteoporosis