Monitoring <i>β</i>-Fructofuranosidase Activity through <i>Kluyveromyces marxianus</i> in Bioreactor Using a Lab-Made Sequential Analysis System
Edwin J. Barbosa-Hernández,
Jorge E. Pliego-Sandoval,
Anne Gschaedler-Mathis,
Javier Arrizon-Gaviño,
Alejandro Arana-Sánchez,
Ricardo Femat,
Enrique J. Herrera-López
Affiliations
Edwin J. Barbosa-Hernández
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Camino Arenero 1227, Col. El Bajío del Arenal., Zapopan C.P. 45019, Mexico
Jorge E. Pliego-Sandoval
Centro Universitario del Sur, Departamento de Ciencias Computacionales e Innovación Tecnológica, Universidad de Guadalajara, Av. Enrique Arreola Silva No. 883, Cólon., Cd. Guzman C.P. 49000, Mexico
Anne Gschaedler-Mathis
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Camino Arenero 1227, Col. El Bajío del Arenal., Zapopan C.P. 45019, Mexico
Javier Arrizon-Gaviño
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Camino Arenero 1227, Col. El Bajío del Arenal., Zapopan C.P. 45019, Mexico
Alejandro Arana-Sánchez
Departamento de Procesos Tecnológicos e Industriales, Instituto Tecnológico y de Estudios Superiores de Occidente, Anillo Perif. Sur Manuel Gómez Morín 8585., San Pedro Tlaquepaque C.P. 45604, Mexico
Ricardo Femat
División de Control y Sistemas Dinámicos, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Lomas 4a Sección., San Luis Potosi C.P. 78216, Mexico
Enrique J. Herrera-López
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Camino Arenero 1227, Col. El Bajío del Arenal., Zapopan C.P. 45019, Mexico
The yeast Kluyveromyces marxianus has shown the potential to produce β-fructofuranosidases, which are enzymes capable of hydrolyzing β-fructofuranosides links of fructans to obtain fructooligosaccharides. The thriving market for fructose syrup and the quality standards imposed by food and pharmaceutical industries have generated an increased search for improved, monitored, and controlled production processes. Monitoring β-fructofuranosidase activity in a bioprocess requires the use of adequate sensors and the processing of information using efficient software algorithms; nevertheless, currently, such a sensor does not exist for this purpose. In this contribution, a sequential injection analysis system (SIA) developed in our laboratory was adapted to monitor at-line β-fructofuranosidase activity produced by the yeast K. marxianus. Samples were taken out automatically from the bioreactor and analyzed using 3,5-dinitrosalicylic (DNS). An algorithm was designed to operate the overall components of the lab-made SIA system. The enzymatic activity error obtained with the automatic SIA compared to the off-line laboratory determinations varied from 0.07% at high enzyme concentrations to 20.39% at low β-fructofuranosidase activity. Further development is required to improve the performance of the lab-made SIA system; nevertheless, such a device must be considered as a potential method for monitoring β-fructofuranosidase activity in real time.
