DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
RALUCA-IOANA TAMPU 1, 2,
ADRIANA FINARU,
CLAIRE ELFAKIR
Affiliations
RALUCA-IOANA TAMPU 1, 2
1. “Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania 2. Institute of Organic and Analytical Chemistry CNRS UMR 7311, University of Orléans, Chartres, BP 6759, 45067 Orléans, France
ADRIANA FINARU
“Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania
CLAIRE ELFAKIR
Institute of Organic and Analytical Chemistry CNRS UMR 7311, University of Orléans, Chartres, BP 6759, 45067 Orléans, France
The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.
