METHODS COMPARISON FOR ENTEROTOXIC STAPHYLOCOCCUS AUREUS CHARACTERIZATION

L. Decastelli; A. Bellio; C. Nogarol; D.M. Bianchi; S. Gallina; T. Brunetto; C. Ghia; M. Gramaglia

doi:10.4081/ijfs.2011.1S.263

Italian Journal of Food Safety (Jan 2011)

METHODS COMPARISON FOR ENTEROTOXIC STAPHYLOCOCCUS AUREUS CHARACTERIZATION

L. Decastelli,
A. Bellio,
C. Nogarol,
D.M. Bianchi,
S. Gallina,
T. Brunetto,
C. Ghia,
M. Gramaglia

Affiliations

L. Decastelli: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta.
A. Bellio: NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino.
C. Nogarol: NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino.
D.M. Bianchi: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta
S. Gallina: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta
T. Brunetto: NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino.
C. Ghia: NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino.
M. Gramaglia: NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino.

DOI: https://doi.org/10.4081/ijfs.2011.1S.263
Journal volume & issue: Vol. 1, no. 1zero
pp. 263 – 264

Abstract

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The aim of this study was to compare two different methods for enterotoxic Staphylococcus aureus characterization. 110 S.aureus strains was isolated from foods and tested with ELISA method able to detect toxins type A to E in culture medium and PCR protocols able to detect the presence of genes (sea to see; seg to sej; sep; ser) encoding for staphylococcal enterotoxins. 27 strains came out positive with ELISA; 68 resulted to have at least one encoding gene. sea and ser genes were detected respectively in 29,1% and 27,3% of strains.

Staphylococcus aureus, enterotoxin, PCR

Published in Italian Journal of Food Safety

ISSN: 2239-7132 (Online)
Publisher: PAGEPress Publications
Country of publisher: Italy
LCC subjects: Technology: Chemical technology: Food processing and manufacture
Website: http://www.ijfs.it

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