Lactoperoxidase Immobilized onto Various Beads for Producing Natural Preservatives Solution

Abstract

The natural preservative agent of hypothiocyanite (OSCN–) has been succeded to determine using immobilized LPO onto various types of beads or matrixes: SP Sepharose Fast Flow (SP-FF), SP Sepharose Big Beads (SP-BB), CNBr activated SP Sepharose (SP-CNBR). Lactoperoxidase (LPO) was obtained from bovine milk. The immobilization method was performed using ion exhange method and adsorption. Immobilized LPO onto matrixes has been stored for 7 days at 4˚C and 10˚C. Data of the production of OSCN– were collected before and after immobilized LPO storage. Data shows no remarkable effect of the production of OSCN– using immobilized LPO stored at 4˚C, however, the remarkable reduction has been detected in the production of OSCN– using immobilized LPO that was stored at 10˚C. As a conclusion, the 7 days storage does not change the capability of immobilized LPO for producing OSCN if the immobilized LPO was stored at 4˚C.

Keywords

• Lactoperoxidase
• immobilization
• storage; hypothiocyanite
• storage
• hyphothiocyanite