Nelumbo nucifera leaf extracts inhibit the formation of advanced glycation end-products and mechanism revealed by Nano LC-Orbitrap-MS/MS

Lu Zhang; Chong-ju Zhang; Zong-cai Tu; Wen-hua Yang; Yi Zhao; Zheng-qi Xin; Hui Wang; Xiao-mei Sha; Juan Chen

Journal of Functional Foods (Mar 2018)

Nelumbo nucifera leaf extracts inhibit the formation of advanced glycation end-products and mechanism revealed by Nano LC-Orbitrap-MS/MS

Lu Zhang,
Chong-ju Zhang,
Zong-cai Tu,
Wen-hua Yang,
Yi Zhao,
Zheng-qi Xin,
Hui Wang,
Xiao-mei Sha,
Juan Chen

Affiliations

Lu Zhang: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
Chong-ju Zhang: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
Zong-cai Tu: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; Corresponding authors at: 99 Ziyang Road, Jiangxi Normal University, Nanchang, Jiangxi, China.
Wen-hua Yang: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China
Yi Zhao: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
Zheng-qi Xin: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
Hui Wang: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; Corresponding authors at: 99 Ziyang Road, Jiangxi Normal University, Nanchang, Jiangxi, China.
Xiao-mei Sha: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
Juan Chen: Key Laboratory of Functional Small Organic Molecule Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China

Journal volume & issue: Vol. 42
pp. 254 – 261

Abstract

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The inhibition of Nelumbo nucifera leaf (NNL) extract and its fractions on the formation of advanced glycation end-products (AGEs) was evaluated with different models. Changes in the conformational structure of bovine serum albumin (BSA) were analyzed by fluorescence spectrometry. Effect of ethyl acetate fraction (EAF) on the glycation sites and glycation degree of BSA was investigated by Nano LC-Orbitrap-MS/MS due to its strongest inhibitory ability. Results indicated that EAF could effectively inhibit the formation of AGEs by scavenging reactive radicals and attenuating the conformational change of BSA induced by glycation. Phenolics and flavonoids were the major contributors. Eleven (11 lysines) and ten (9 lysines and 1 arginine) glycation sites were identified from glycated BSA with and without EAF, respectively. Addition of EAF could not reduce the number of glycation sites of BSA, but could significantly decrease the glycation degree of most glycated peptides and alter the location of glycation.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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