Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis

Shan Tang; Yun-yi Hou; Rui-yi Hu; Jun-jie Zhang; Xu-fei Gao; Ming Zhang; Zi Wang; Rui Zhang; Pin-hui Jia; Wei Li

Journal of Functional Foods (Apr 2024)

Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis

Shan Tang,
Yun-yi Hou,
Rui-yi Hu,
Jun-jie Zhang,
Xu-fei Gao,
Ming Zhang,
Zi Wang,
Rui Zhang,
Pin-hui Jia,
Wei Li

Affiliations

Shan Tang: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China
Yun-yi Hou: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Rui-yi Hu: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Jun-jie Zhang: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Xu-fei Gao: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Ming Zhang: College of Medicine, Jilin University, Changchun 130021, China
Zi Wang: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China
Rui Zhang: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Pin-hui Jia: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Wei Li: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.

Journal volume & issue: Vol. 115
p. 106102

Abstract

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Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's anti-aging properties remains unclear. This study established an in vitro D-galactose-induced N2a cells subacute aging model to investigate AFG's anti-aging activity and potential mechanisms. Results indicated that AFG treatment (8 μM) significantly reduces the overexpression of aging-related proteins (p53/p21/p16) induced by D-galactose, highlighting its anti-aging efficacy. AFG concurrently diminishes ROS accumulation, lowering extracellular lactate dehydrogenase and alleviating intracellular oxidative stress. Notably, AFG suppresses the PERK/CHOP/caspase 12 pathway, reducing intracellular Ca2+ concentration and mitigating endoplasmic reticulum (ER) stress. Additionally, AFG decreases Bax and caspase 3 expression, elevates Bcl-2 and mitochondrial membrane potential, mitigating apoptosis. NAC with 4-PBA validated the results. Overall, AFG protects N2a cells from aging by reducing ER stress and apoptosis, suggesting its potential as a key substance for treating aging and neurodegenerative diseases.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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