Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum

Abstract

Brown seaweed, which is an abundant source of secondary metabolites, contains high levels of sulfated polysaccharides and possesses various biological properties. Here, we aimed to determine the chemical characteristics and evaluate the potential lipid-lowering effects of sulfated polysaccharides from Sargassum coreanum (SPSC) on differentiated 3T3-L1 cells. Treatment of differentiated 3T3-L1 cells with MCS markedly reduced the intracellular lipid accumulation by over 50% at 200 μg/mL of MCS. In addition, the key adipogenic peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, lipogenic sterol regulatory element-binding protein 1, lipolytic adipose triglyceride lipase, and AMP-activated protein kinase were identified in 3T3-L1 cells. In the in vivo study, alamarBlue staining showed that energy expenditure was significantly (* p < 0.05, *** p < 0.01) increased in MCS-treated zebrafish embryos. These findings suggested that the crude polysaccharides from Sargassum coreanum possesses intracellular lipid-inhibitory activity and could be utilized as a candidate for lipid-lowering functional food products.

Keywords

• Lipid lowering activity
• Sargassum coreanum
• Sulfated polysaccharides
• Lipogenesis
• Adipogenesis
• 3T3-L1