Effect of functional beetroot pomace biscuit on phenylhydrazine induced anemia in albino rats: Hematological and blood biochemical analysis

Abstract

The present study incorporated beetroot pomace powder in biscuit preparation at concentrations of 5–20%. Biscuits' chemical composition, and sensory were evaluated. A feeding experiment was conducted using three groups (n = 3); control and anemic (An+) groups fed a standard diet, and anemic group fed 90% standard-diet and 10% biscuit containing 15% pomace (AnB). Anemia induced by injecting rats with phenylhydrazine (40 mg/kg) for two consecutive days. Hematological and biochemical blood parameters were determined at days 3, 14, and 28. An enhancement in protein, fiber, calcium, phosphorus, and iron was observed in the biscuit by increasing the pomace. Consuming biscuits containing 15% pomace increased hemoglobin, RBCs, and antioxidant enzymes in (AnB) rats after 28 days. The minor kidney and liver dysfunction in the anemic groups were recovered in (AnB) after 28 days. Consequently, incorporation beet pomace in biscuit revealed a promising role in anemia treatment and oxidative stress managing.

Keywords

• Anemia
• Beetroot
• Pomace
• Phenylhydrazine
• Hematological analysis
• Blood biochemical analysis