Biofilm Inhibition, Antibacterial and Antiadhesive Properties of a Novel Biosurfactant from <i>Lactobacillus paracasei</i> N2 against Multi-Antibiotics-Resistant Pathogens Isolated from Braised Fish
Hippolyte Tene Mouafo,
Alphonse Tegang Sokamte,
Linda Manet,
Arsene Joseph Manga Mbarga,
Sachivkina Nadezdha,
Somashekhar Devappa,
Augustin Mbawala
Affiliations
Hippolyte Tene Mouafo
Centre for Food, Food Security and Nutrition Research, Institute of Medical Research and Medicinal Plants Studies, Yaoundé P.O. Box 13033, Cameroon
Alphonse Tegang Sokamte
Department of Food Engineering and Quality Control, University Institute of Technology, University of Ngaoundéré, Ngaoundéré P.O. Box 455, Cameroon
Linda Manet
Centre for Food, Food Security and Nutrition Research, Institute of Medical Research and Medicinal Plants Studies, Yaoundé P.O. Box 13033, Cameroon
Arsene Joseph Manga Mbarga
Department of Microbiology V.S. Kiktenko, Medical Institute, RUDN University Named after Patrice Lumumba, 119435 Moscow, Russia
Sachivkina Nadezdha
Department of Microbiology V.S. Kiktenko, Medical Institute, RUDN University Named after Patrice Lumumba, 119435 Moscow, Russia
Somashekhar Devappa
Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysore 570020, Karnataka, India
Augustin Mbawala
Department of Food Sciences and Nutrition, National School of Agro-Industrial Sciences, University of Ngaoundéré, Ngaoundéré P.O. Box 455, Cameroon
This study aimed to assess the antibiotic susceptibility and biofilm formation ability of pathogens isolated from braised fish as well as characterize and evaluate the antibacterial, antiadhesive, and antibiofilm activities of the biosurfactant from Lactobacillus paracasei subsp. tolerans N2 against these pathogens. The susceptibility of six pathogens isolated from braised fish (Escherichia coli EM2, Staphylococcus aureus SA1, Salmonella enteritidis PE1, Pseudomonas aeruginosa CT3, Yersinia enterolitica MH5, Proteus mirabilis MR2, and Klebsiella pneumoniae AG5) to 16 antibiotics revealed multiple resistances with an MAR index greater than 0.3. These pathogens were able to form biofilms with S. aureus SA1, which showed the highest ability. Using biochemical and elemental analyses, FTIR, GC-MS, 1H NMR and high-resolution mass spectrometry, the biosurfactant was characterized as a novel glycolipoprotein made of two congeners of mass 482.28 and 507.27 m/z, respectively. They showed bactericidal and antiadhesive activities against all pathogens. The biosurfactant inhibited biofilm formation by these pathogens and eradicated mature biofilms.
