Biocontrol Potential of Novel Emerging Multiacting Bacterium <i>Bacillus amyloliquefaciens</i> NC6 against <i>Brevicoryne brassicae</i> in <i>Brassica rapa</i> ssp. <i>Pekinensis</i>

Abstract

The emerging elicitor protein PeBA1, extracted from Bacillus amyloliquefaciens NC6, was tested against the cabbage aphid (Brevicoryne brassicae) for its biocontrol potential. Its effects on the survival, lifespan, immature development, adult reproductive performance, and pest–pathogen interaction were assessed using electrical penetration graphs (EPGs) against B. brassicae. Furthermore, the direct effects of PeBA1 with temperature and climate change in Brassica rapa ssp. Pekinensis plants were investigated by the characterization of active compounds in B. amyloliquefaciens with multi-acting entomopathogenic effects. Compared with controls, PeBA1 treatments decreased (second- and third-generation) B. brassicae population growth rates. In a host selection test, control plants were colonized faster by B. brassicae than PeBA1-treated B. rapa plants. The B. brassicae nymphal development was extended by PeBA1 concentrations. Likewise, fecundity was reduced in PeBA1-treated seedlings compared with control, with fewer offspring produced. The trichomes and wax production on PeBA1-treated leaves resulted in a hostile environment for B. brassicae. PeBA1 altered the surface structure of the leaves, reducing B. brassicae reproduction and preventing colonization. Systemic defensive processes also included the activation of pathways (JA, SA, and ET). Based on these findings against B. brassicae, integrated pest management and bio control with PeBA1 in the agroecosystem appears to be suitable.

Keywords

• <i>Bacillus amyloliquefaciens</i>
• PeBA1
• <i>Brevicoryne brassicae</i>
• <i>Brassica rapa</i> ssp. <i>Pekinensis</i> larval instars
• fecundity
• gene expression