Uncovering the Effects of the Cultivation Condition on Different Forms of Peptaibol’s Emericellipsins Production from an Alkaliphilic Fungus, <i>Emericellopsis alkalina</i>
Anastasia E. Kuvarina,
Maxim A. Sukonnikov,
Alla V. Timofeeva,
Marina V. Serebryakova,
Ludmila A. Baratova,
Mikhail N. Buzurnyuk,
Alexander V. Golyshkin,
Marina L. Georgieva,
Vera S. Sadykova
Affiliations
Anastasia E. Kuvarina
Department of Microbiology, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia
Maxim A. Sukonnikov
Department of Microbiology, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia
Alla V. Timofeeva
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Marina V. Serebryakova
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Ludmila A. Baratova
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Mikhail N. Buzurnyuk
Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
Alexander V. Golyshkin
Department of Microbiology, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia
Marina L. Georgieva
Department of Microbiology, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia
Vera S. Sadykova
Department of Microbiology, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia
Peptaibols (Paib) are a class of biologically active peptides isolated from fungi and molds, which have attracted the attention of medicinal chemists due to their widely ranging pharmacological properties, including their antimicrobial activity. In the present study, we investigated the effects of various pH levels and cultivation conditions on peptaibol complex emericellipsins A-E (EmiA-E), produced by the alkaliphilic fungus Emericellopsis alkalina. Paib production has been studied in flasks and bioreactors for different pH values ranging from 7 to 11. The study of morphological features based on light and scanning electron microscopy has revealed differences between fungi grown at different pH values and cultivation conditions. Emericellipsins have been purified, sequenced, and identified by mass spectrometry. We have found that an alkaline pH of 10 could promote emericellipsins’ biosynthesis most effectively during stationary fermentation on the 14th day of cultivation.