Chlorogenic acid enhances intestinal barrier by decreasing MLCK expression and promoting dynamic distribution of tight junction proteins in colitic rats

Zheng Ruan; Shumei Mi; Lili Zhou; Yan Zhou; Jing Li; Wenhui Liu; Zeyuan Deng; Yulong Yin

Journal of Functional Foods (Oct 2016)

Chlorogenic acid enhances intestinal barrier by decreasing MLCK expression and promoting dynamic distribution of tight junction proteins in colitic rats

Zheng Ruan,
Shumei Mi,
Lili Zhou,
Yan Zhou,
Jing Li,
Wenhui Liu,
Zeyuan Deng,
Yulong Yin

Affiliations

Zheng Ruan: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Shumei Mi: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Lili Zhou: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Yan Zhou: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Jing Li: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Wenhui Liu: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
Zeyuan Deng: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China; Corresponding authors. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China. Fax: +86 791 88304402.
Yulong Yin: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China; Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Corresponding authors. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China. Fax: +86 791 88304402.

Journal volume & issue: Vol. 26
pp. 698 – 708

Abstract

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The aim of the present study was to evaluate the effect of chlorogenic acid (CGA) on the mucosal barrier function and tight junction proteins in colitic rats. CGA (60 mg/kg body weight) significantly decreased the ratio of lactulose to mannitol (L/M) (P < 0.05), and endotoxin level (P < 0.01) in CGA + colitis group, compared to colitis group. CGA supplementation in CGA + colitis group increased the expression of occludin (P < 0.05), claudin-1 (P < 0.01), ZO-1 (P < 0.05), decreased the expression of myosin light chain kinase (MLCK), compared to colitis group. CGA also increased the phosphorylation level of occludin (P < 0.01) at tight junction, and decreased the phosphorylation level of occludin (P < 0.05) and claudin-1 (P < 0.05) on the basolateral membranes. These results indicate that CGA supplementation attenuates colonic barrier damage by decreasing MLCK expression and promoting molecular dynamic distribution of tight junction proteins in colitic rats.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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