Wasabi leaf supplementation had antioxidant, anti-glycation, and improved skin melanin, spot and moisture

Abstract

The beauty of nature led to the rise of the human medical cosmetic industry, which gave a breed between beauty care products and pharmaceutical, medical-stressed skin care and beauty care products. However, the current clinical research on wasabi leaves and skin is still unclear. The purpose of this study was to investigate the wasabi by-product-wasabi leaves, for antioxidant, anti-glycation and whitening effects. This study used wasabi leaf extract treated in melanocytes, and examined melanin, inflammatory factors, advanced glycation end products (AGEs), and recruited subjects to take wasabi leaf extract beverage labeled 50 mL or a placebo drink daily for 8 weeks. The results showed that wasabi leaf extract can significantly inhibit tyrosinase activity by 24 %, decrease melanin content by 21 %, decrease IL-8 gene expression by 74.2 % and inhibit the AGEs formation by 59 % compared to the control group in vitro. In clinical trial, wasabi leaf extract significantly increased total antioxidant capacity by 7.9 %, and decreased AGEs by 5.6 %, and decreased skin melanin, spots, deep spots by 4.0 %, 3.2 %, 4.2 %, increased skin moisture by 6.6 % compared to placebo group. The wasabi leaf extract decreased tyrosinase activity, melanin content, AGEs formation in vitro, and improved skin condition in clinical trial. The wasabi leaves could be regarded as an unexplored natural source of bioactive ingredients and applied for the development of related value-added products.

Keywords

• Antioxidant
• Advanced glycation end products
• Wasabi leaf
• Skin
• Melanin