Aronia juice improves working memory and suppresses δ-secretase activity in 5XFAD mice
Takuya Yamane,
Momoko Imai,
Satoshi Handa,
Hideo Ihara,
Tatsuji Sakamoto,
Tetsuo Ishida,
Takenori Nakagaki,
Susumu Uchiyama
Affiliations
Takuya Yamane
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan; Department of Biofunctional Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Sakai, Osaka 599-8531, Japan; Institute of Metabolomics, BYU-Analytica Inc., Suita, Osaka 565-0871, Japan; Corresponding author at: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Momoko Imai
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan; Institute of Metabolomics, BYU-Analytica Inc., Suita, Osaka 565-0871, Japan
Satoshi Handa
Department of Biofunctional Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Sakai, Osaka 599-8531, Japan
Hideo Ihara
Department of Biological Science, Graduate School of Science, Osaka Metropolitan University, Sakai, Osaka 599-8531, Japan
Tatsuji Sakamoto
Department of Biofunctional Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Sakai, Osaka 599-8531, Japan
Tetsuo Ishida
Department of Nutrition, Koshien University, Takarazuka, Hyogo 665-0006, Japan
Takenori Nakagaki
Nakagaki Consulting Engineer and Co., Ltd, Nishi-Ku, Sakai 593-8328, Japan
Susumu Uchiyama
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan; Institute of Metabolomics, BYU-Analytica Inc., Suita, Osaka 565-0871, Japan
Asparagine endopeptidase (AEP) cleaves amyloid precursor protein to facilitate amyloid β (Aβ) production in the brain. Aronia berry (Aronia melanocarpa) has various beneficial effects on health through inhibition of enzymes such as dipeptidyl peptidase IV. In this study, to examine neuroprotective effects of aronia juice (AJ), AJ was given to male 5XFAD Alzheimer's disease model mice via drinking bottles over a period from 2 weeks to 12 weeks of age. AJ intake improved the performance of Y-maze test in 5XFAD mice, and suppressed the δ-secretase activity in the mouse brains. AJ intake reduced significantly Aβ deposition in the brain. AJ reduced δ-secretase activity in human neuroblastoma SH-SY5Y cells. Out of five fractions (F1–F5) obtained from AJ, only F5 reduced δ-secretase activity in SH-SY5Y cells in a dose-dependent manner. LC-MS/MS analysis suggested that F5 contains three quercetin glycosides. AJ has neuroprotective function through inhibition of δ-secretase activity of AEP.
