Identification of Chemical Components in Gannan Navel Orange Pulp by UPLC-QTOF-MS Coupled with HS-SPME-GC-MS

Abstract

Using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS), we separated and identified 148 non-volatile organic compounds including flavonoids, organic acids and amino acids in navel orange pulp in 37 min. The volatile organic compounds (VOCs) in the pulp of navel oranges from four major production regions in southern Jiangxi Province, Ruijin, Xinfeng, Anyuan and Nankang were detected by headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS). In total, 97 VOCs were identified including esters, alcohols, aldehydes and terpenes. The results of this study provide detailed data for further research on chemical substances in the pulp of Gannan navel oranges.

Keywords

• ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; headspace solid-phase microextraction-gas chromatography-mass spectrometry; gannan navel oranges; volatile organic compounds; non-volatile organic components