Dietary tea tree (Melaleuca alternifolia) oil supplementation enhances the expressions of amino acid transporters in goat ileal mucosa and improves intestinal immunity

Xiaokang Lv; Liang Chen; Chuanshe Zhou; Yibing Guo; Guijie Zhang; Jinhe Kang; Zhiliang Tan; Shaoxun Tang; Zixin Liu

doi:10.1002/fsn3.2972

Food Science & Nutrition (Nov 2022)

Dietary tea tree (Melaleuca alternifolia) oil supplementation enhances the expressions of amino acid transporters in goat ileal mucosa and improves intestinal immunity

Xiaokang Lv,
Liang Chen,
Chuanshe Zhou,
Yibing Guo,
Guijie Zhang,
Jinhe Kang,
Zhiliang Tan,
Shaoxun Tang,
Zixin Liu

Affiliations

Xiaokang Lv: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Liang Chen: Shenyang Agricultural University Institute of Rural Revitalization Strategy Shenyang China
Chuanshe Zhou: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Yibing Guo: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Guijie Zhang: School of Agriculture Ningxia University Yinchuan China
Jinhe Kang: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Zhiliang Tan: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Shaoxun Tang: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Zixin Liu: CAS Key Laboratory for Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China

DOI: https://doi.org/10.1002/fsn3.2972
Journal volume & issue: Vol. 10, no. 11
pp. 3749 – 3758

Abstract

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Abstract Tea tree oil (TTO) is a plant‐derived additive with anti‐inflammatory, bactericidal, and growth‐promoting properties. However, little is known about the effects of TTO on intestinal amino acid transport and immune function in goats. Twenty‐four Ganxi goats (initial body weight of 13.5 ± 0.70 kg) were randomly allotted two treatments and fed either control (CON) or CON+TTO (0.2 ml/kg) diet. The addition of TTO to the diet significantly decreased (p < .05) tumor necrosis factor‐α content and increased (p < .05) interleukin‐2 (IL‐2) content in goat serum; significantly decreased (p < .05) IL‐12, and increased (p < .05) IL‐2 content in goat ileal mucosa; significantly increased (p < .05) secreted IgA content in the jejunal and ileal mucosa; significantly upregulated (p < .05) IL‐2 and downregulated (p < .05) IL‐12 at the mRNA level in the ileal mucosa; significantly elevated the levels of serine, arginine, and total amino acids in the ileal mucosa (p < .05); significantly upregulated (p < .05) SLC1A1 and SLC7A1 in the ileum; and significantly enhanced (p < .05) the protein expression of Claudin‐1 in the ileal mucosa. In summary, adding 0.2 ml/kg of TTO to the diet enhanced SLC1A1 and SLC7A1 mRNA expression in the ileal mucosa, and SLC1A1 and SLC7A1 could transport serine and arginine from the chyme to the ileal mucosa. Thus, increased serine and arginine content in the mucosa could improve intestinal immunity. TTO supplementation upregulated the expression of IL‐2 and Claudin‐1 in goat ileal mucosa, and enhanced immune function in the intestine.

Published in Food Science & Nutrition

ISSN: 2048-7177 (Online)
Publisher: Wiley
Country of publisher: United States
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://onlinelibrary.wiley.com/journal/20487177

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