Biosynthesis of Glucaric Acid by Recombinant Strain of <i>Escherichia coli</i> Expressing Two Different Urinate Dehydrogenases
Xinchao Yang,
Linlin Niu,
Chunjiang Ye,
Yuanxiu Wang,
Yuehui Liu,
Fang Wang,
Naxin Sun
Affiliations
Xinchao Yang
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
Linlin Niu
Shandong Provincial Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Postdoctoral Scientific Research Workstation, Shandong Academy of Pharmaceutical Sciences, Jinan 250101, China
Chunjiang Ye
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
Yuanxiu Wang
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
Yuehui Liu
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
Fang Wang
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
Naxin Sun
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
D-glucaric acid is an important bio-based building block of polymers and is a high value-added chemical that can be used in a variety of applications. In the present study, the Udh target genes from Pseudomonas putida and Pseudomonas syringae were used together to construct the expression vector pETDuet-2 × Udh. The transformants of BL21 (DE3) with vector pETDuet-2 × Udh were applied to produce glucaric acid from glucuronic acid. After optimizing the induction conditions, the highest Udh expression was achieved when 0.4 mmol·L−1 isopropyl-β-d–thiogalactoside (IPTG) was added to the cell cultures at an OD600 value of 0.6 followed by culturing at 26 °C for 6 h. The production of glucaric acid substantially reached 5.24 ± 0.015 g·L−1 in fed-batch cultures in a 30 L tank. In the present study, a new system for glucaric acid production was established, which was more economic and friendly to the environment.
