Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model

Abstract

In this study, the main components of the extract of (Lithocarpus litseifolius folium extract, LLFE) were analyzed,and the anti-oxidative stress and anti-aging effects of LLFE on C.elegans were evaluated using Caenorhabditis elegans (C.elegans) as a model to explore the possible mechanisms of action.The results showed that LLFE contained 52.84±1.45 mg/g of total phenols, 380.74±15.12 mg/g of total flavonoids, 470.36±5.84 mg/g of total sugars and about 6.00% of dihydrochalcone compounds, with phloridzin 52.76±0.71 mg/g, phloretin 1.64±0.06 mg/g and trilobatin 4.88±0.01 mg/g. LLFE (0.25, 1.25 mg/mL) also significantly extended the mean lifespan of C.elegans under oxidative and heat stress conditions (P<0.05) and enhanced their own resistance to stress. After using H2O2-induced oxidative damage in C.elegans as a model, it was found that LLFE (0.05, 0.25 and 1.25 mg/mL) significantly increased the mean lifespan of C.elegans compared with the model group (P<0.05). When the LLFE concentration was 1.25 mg/mL, the total superoxide dismutase activity in C.elegans increased by 39.85% (P<0.05), catalase activity by 39.21%(P<0.05) and glutathione peroxidase activity by 66.95% (P<0.001). The accumulation of malondialdehyde and reactive oxygen species was also reduced by 66.05% (P<0.001) and 49.69% (P<0.001), respectively. This indicates that LLFE has good antioxidant effect, and then delay the process of aging.

Keywords

• dihydrochalcone
• caenorhabditis elegans
• lifespan
• oxidative stress
• lithocarpus polystachy folium