Structural characterization and anti-hypoxia activities of polysaccharides from the sporocarp, fermentation broth and cultured mycelium of Agaricus bitorquis (Quél.) Sacc. Chaidam in mice

Abstract

Three kinds of water-soluble polysaccharides (PS, PFB and PM) were isolated from the sporocarp, fermentation broth and cultured mycelium of wild mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam (ABSC). The Mw of PS, PFB and PM ranged from 893 Da to 38,340 Da. FTIR and NMR spectroscopy indicated that the skeleton of PS-I in PS contained α-D-mannopyranose with a backbone of (1 → 4)-α-D-glycosidic. The isolated fraction PFB-I in PFB was connected by (1 → 6) or (1 → 4)-α-D-glycosidic linkage. PM-I in PM was connected by (1 → 4)-α-D-glucopyranosyl residues with branched chain connected by (1 → 6)-α-glycosidic linkage. The anti-hypoxia activity results in vivo revealed that the supplementation with PS, PFS and PM could extend the survival time of mice under hypoxia stress, and increase GSH-Px, CAT and SOD activities and protein levels in liver tissues of mice, decrease MDA and NO levels. The findings suggested that such polysaccharides from ABSC showed anti-hypoxia activity.

Keywords

• Agaricus bitorquis (Quél.) Sacc. Chaidam
• Polysaccharides
• Structural characterization
• Anti-hypoxia activity