α-Amylase Production by Bacillus cereus MTCC 1305 Using Solid-State Fermentation

Abstract

Production of α-amylase under solid-state fermentation by Bacillus cereus MTCC 1305 has been investigated using wheat bran and rice flake manufacturing waste as substrates. With wheat bran, highest enzyme production expressed as units per mass of dry substrate ((94±2) U/g) was observed. Production parameters were optimized as inoculum size 10 % (volume per mass) and substrate:moisture ratio 1:1. Among different carbon sources supplemented, glucose (0.04 g/g) showed enhanced enzyme production ((122±5) U/g). Supplementation of different nitrogen sources (0.02 g/g) showed decline in enzyme production. Optimum α-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C, enzyme showed 90 % activity compared to 55 °C.

Keywords

• α-amylase
• Bacillus cereus
• solid-state fermentation
• wheat bran
• optimization