Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods

Abstract

In this paper, the polysaccharides fingerprints of Polygonatum cyrtonema Hua. were constructed, combined with various chemometric methods, to evaluate the differences of P. cyrtonema polysaccharides from different origins. The 1-pheny-3-methyl-5-pyrazolone (PMP) pre-column derivatization-HPLC method was applied to analyze the monosaccharide composition and total polysaccharide content of P. cyrtonema. Furthermore, the fingerprints were further evaluated by chemometric methods, including similarity analysis (SA), cluster analysis (HCA), and principal component analysis (PCA). The results showed that the composition of monosaccharides was galacturonic acid, glucuronic acid, galactose, glucose, and xylose. The similarities were ranged from 0.781 to 0.945. In addition, the content of total polysaccharides was in the range of 7.18% to 16.27%. HCA and PCA all divided P. cyrtonema polysaccharides from different origins into two categories, the samples collected in Cili County, Hunan Province were a separate one. These results indicated that the polysaccharide fingerprints of P. cyrtonema were quite different. Establishing the standardized cultivation technology system of P. cyrtonema is necessary to ensure clinical efficacy and safety.

Keywords

• pre-column derivatization
• polygonatum cyrtonema
• polysaccharide
• quality evaluation
• fingerprint
• chemometrics