Immobilization of Saccharomyces Cerevisiae on Alumina Ceramic Beads In Order to Reduce Aflatoxin M1 In Vitro

Abstract

In this study the ability of Saccharomyces cerevisiae PTCC 5052 to adsorption of aflatoxin M1 was assessed. In order to improve operational efficiency, Alumina ceramic was analyzed as a potential support for immobilization of yeast cells. In immobilization process, results indicated that the binding abilities of AFM1 by live cells of Saccharomyces cerevisiae were higher than non-live immobilized cells on alumina ceramic within 48 hours (P1 solution (0.2 ppb) was passed through a ceramic substrate containing immobilized saccharomyces cerevisiae (both of live and non-live immobilized cells) in 5, 10 and 20 minutes. The results showed that the residual aflatoxin M1 in the solution after 20 minutes of circulation was minimal and the highest percentage of AFM1 reduction was 75. Alumina beads containing live immobilized yeast cells compared non- live yeasts, significantly reduced aflatoxin M1. The results of this study showed that the alumina ceramic can be used as a suitable bed for immobilization of saccharomyces cerevisiae to remove aflatoxin M1.

Keywords

• aflatoxin
• alumina
• immobilization
• saccharomyces cerevisiae