Effect of Lactobacillus rhamnosus LZ260E on allergic symptoms and intestinal microbiota in β-lactoglobulin–sensitized mice

Abstract

β-lactoglobulin (β-Lg) in milk is the main allergen that causes intestinal inflammation. Our previous study reported that Lactobacillus rhamnosus LZ260E (LZ260E) showed potential anti-allergic activity. However, the specific mechanism by which LZ260E exerts its anti-allergic activity remains unclear. This study investigated the effect of LZ260E on β-Lg-induced milk allergen mice model. The results showed that oral administration of LZ260E reduced spleen and thymus indices in mice. In addition, oral administration of LZ260E decreased the serum levels of IgE, histamine, IL-4, and monocyte chemotactic protein-1 (MCP-1), upregulated the expression of IFN-γ, IL-10, TNF-α, and TGF-β, and repaired colon damage. It was also found that LZ260E regulated alterations in the intestinal microbiota caused by allergies. These results showed that LZ260E has anti-allergic properties by regulating the gut microbiota and Th1/Th2 immunological balance, implying that it may be a functional probiotic against cow's milk caused allergies.

Keywords

• Lactobacillus rhamnosus
• Anti-allergic
• β-lactoglobulin
• Intestinal microbiota