A versatile functional food source Lasia spinosa leaf extract modulates the mRNA expression of a set of antioxidant genes and recovers the paracetamol-induced hepatic injury by normalizing the biochemical and histological markers

Abstract

Lasia spinosa leaf extract (LSML) has been studied as a functional food source for DNA damage protection and antioxidant gene expression in paracetamol (PCM)-induced liver injury. The DNA damage-protecting capacity of LSML was evaluated using pBR322 plasmid DNA. A toxic dose of PCM was administered to rats, followed by an intervention with LSML of 65–250 mg/kg for 22 days. In LSML-treated animals, a significant decrease in hepatic parameters, hepatocyte growth, and cell injury was noticed when compared to the hepatic control. The increase of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and decrease of malondialdehyde (MDA) were manifested. The serum total protein, ALP, ALT, AST, and bilirubin reverted to normal compared to the healthy and silymarin-treated groups. An elevated mRNA expression of SOD2, CAT, GPX1, PON1, and PFK1 was manifested in LSML-treated groups. The results demonstrate that LSML could be an antioxidant gene upregulating functional food sources to protect against liver injury.

Keywords

• LSML
• pBR322
• SOD2
• CAT
• GPX1
• PON1