Polysaccharides from Dendrobium officinale inhibit proliferation of osteosarcoma cells and enhance cisplatin-induced apoptosis

Abstract

Dendrobium officinale polysaccharides (DOPs) are carbohydrates predominantly composed of mannose (M1) and glucose, and are known to exhibit antitumour activities. Here, the antitumour properties of carbohydrates including monosaccharide M1, oligosaccharide mannohexaose (M6), and DOPs were investigated. Polysaccharides DOP122, DOP20, DOP8, and DOP2 with molecular weights of 1224.54, 200.99, 80.32, and 24.89 kDa, respectively, were obtained from Dendrobium officinale. Among M1, M6, and DOP122-DOP2, DOP20 exerted the strongest inhibitory effect on the proliferation of osteosarcoma (OS) U2OS and Saos-2 cells. When combined with cisplatin (DDP), DOP20 showed a synergistic inhibition of proliferation. Moreover, DOP20 enhanced DDP-induced cell apoptosis mediated by the mitochondrial pathway by up-regulating P53, Bax, and Bak expression; down-regulating Bcl-2 and Mcl-1 expression; and increasing Cleaved caspase9/Caspase9, Cleaved caspase3/Caspase3, and Cleaved PARP/PARP ratios. These findings confirmed that carbohydrate drugs, especially polysaccharides, used in the treatment of tumours need to be validated in terms of a suitable molecular weight.

Keywords

• Dendrobium officinale polysaccharide
• Mannose
• Osteosarcoma
• Cisplatin
• Proliferation
• Apoptosis