Italian Journal of Food Safety (Nov 2020)

Application of the Micro Biological Survey analytical method for the determination of bacterial load in cow raw milk

  • Alessandra Cornacchia,
  • Maria Antonietta Saletti,
  • Violeta Di Marzio,
  • Romolo Salini,
  • Cristina Marfoglia,
  • Elga Tieri,
  • Nicola D’Alterio,
  • Nicla Marri,
  • Francesca Losito,
  • Alyexandra Arienzo,
  • Lorenza Murgia,
  • Giovanni Antonini,
  • Simonetta Amatiste,
  • Loris Leboffe,
  • Francesco Pomilio

DOI
https://doi.org/10.4081/ijfs.2020.7696
Journal volume & issue
Vol. 9, no. 3

Abstract

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The aim of this study was to evaluate the performance of “Micro Biological Survey – MBS Test” in the enumeration of bacterial load in cow raw milk. The MBS test is based on a colorimetric method recently developed and patented by “Roma Tre” University, Italy. The evaluation of the performance of the MBS method was carried out by comparison with plate count at 30°C (gold standard) and flow cytometry. Thirteen independent set of experiments were performed analyzing a total of 104 samples of cow raw milk with the selected methods. Results obtained using the MBS method are comparable with those obtained with the plate count method at 30°C (CFU/mL) and flow cytometry technology; in particular, the results obtained with the MBS method are very close to plate count’s at 30°C. On the other hand, there are statistically significant differences between these two methods’ and flow cytometry technology’s results that could be due to the different experimental conditions.

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