Journal of Acute Disease (Jan 2020)

Identification of Panton-Valentine leukocidin virulence gene in methicillin—resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran

  • Atefeh Ranjkesh,
  • Zahra Salari,
  • Mehrab Nejat,
  • Emad Behboudi,
  • Ali Ramezani,
  • Milad Zandi,
  • Mahmood Marzban,
  • Hadis Kiani,
  • Mina Bavi,
  • Mehdi Rabiei Roodsari,
  • Yousef Esvand Zibaee,
  • Samaneh Abbasi,
  • Maryam Mohammadi Elyasi,
  • Mehdi Parsanahad,
  • Shahram Jalilian,
  • Simin Mohammadi Gorji,
  • Mina Owrang

DOI
https://doi.org/10.4103/2221-6189.283890
Journal volume & issue
Vol. 9, no. 3
pp. 121 – 125

Abstract

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Objective: To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital. Methods: A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017. All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles, and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies. Then the presence of the Panton-Valentine leukocidin gene was tested by PCR. Results: Of the methicillin-resistant Staphylococcus aureus samples, 80% were negative for the Panton-Valentine leukocidin gene, and only 20% of the samples had Panton-Valentine leukocidin gene. Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene (16.12% vs. 33.33%) (P=0.25). Besides, there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene. Conclusions: In recent years, increased resistance has been a serious threat. The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.

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