Protective effects of extracellular proteins of Saccharomycopsis fibuligera on UVA-damaged human skin fibroblasts

Abstract

Saccharomycopsis fibuligera is recognized as a food-based yeast which is widely used in food and drugs due to its high yield characteristics of extracellular enzymes. In this study, the extracellular proteins of S. fibuligera (SF-1-ECPs) are analyzed by HPLC, finding that 22.24% of the molecular weight of SF-1-ECPs is less than 2000 Da, and biochemical experiments, cell and cell molecular experiments are conducted to explore the significance of SF-1-ECPs in cosmetics. The results show that SF-1-ECPs can enhance antioxidant enzyme activity and mRNA expression within cells by using the Nrf2/Keap1/Mafk pathway to eliminate intracellular reactive oxygen, thereby protecting human skin fibroblasts from UVA-induced oxidative stress. They can also protect COL-I degradation caused by the increase of MMP-1 and thus delay skin aging.

Keywords

• Saccharomycopsis fibuligera
• Fungal proteins
• Oxidative stress
• NF-E2-related factor 2
• Skin aging