Study on the Enrichment of Peptides in Wheat Germ by Microwave Combined with Metal Ions

Abstract

In order to efficiently enrich wheat germ polypeptide, microwave combined with metal ions were used to treat wheat germ, and the effects of microwave power, microwave time, ZnSO4 concentration, MnSO4 concentration, and CaCl2 concentration on wheat germ peptide content and protease activity were investigated. The process parameters were optimized by orthogonal experiment on the basis of single factor. The results showed that under the conditions of microwave power 400 W, microwave time 10 s, ZnSO4 concentration 1.0 mmol/L, MnSO4 concentration 1.6 mmol/L and CaCl2 concentration 1.4 mmol/L, the polypeptide content in wheat germ could reach 228.85 mg/g, and the protease activity increased to 2268.40 U/g. Microwave combined with metal ions can significantly promote the enrichment of peptides in wheat germ.

Keywords

• wheat germ
• metal ion
• microwave
• peptide content
• protease activity