Shipin Kexue (Jun 2023)

Comparison of Four Methods for the Determination of the Amylose Content in Foxtail Millet

  • LIU Jianlei, SHANG Bo, XING Xiaoting, ZHANG Dong, CHANG Liu, SUN Hui, DUAN Xiaoliang

DOI
https://doi.org/10.7506/spkx1002-6630-20220719-216
Journal volume & issue
Vol. 44, no. 12
pp. 217 – 224

Abstract

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In this study, the amylose contents in 24 commercial foxtail millet samples from major production provinces in China were determined by four different methods, namely, the improved national standard method (GB/T 15683-2008), the spectrophotometric method (NY/T 2639-2014) recommended by China’s agricultural authority, the dual-wavelength method, and the concanavalin A (Con A) method. The results of these methods were in the following decreasing order: improved GB/T 15683-2008 ≈ dual-wavelength > NY/T 2639-2014 > Con A. The correlation coefficient between the results of improved GB/T 15683-2008 and those of NY/T 2639-2014 was highest, 0.752 (P = 0), the former being about 1.41 times higher than the latter, which could allow simple prediction of the amylose content of foxtail millet. In conclusion, the improved GB/T 15683-2008 method, the dual-wavelength method and the NY/T 2639-2014 (× 1.41) method can be used to accurately determine the content of amylose in foxtail millet while greatly reducing sample testing time.

Keywords