Improvement of the Nutritional Quality of <i>Psophocarpus tetragonolobus</i> Tubers by Fermentation with Ruminal Crabtree-Negative Yeasts on the In Vitro Digestibility and Fermentation in Rumen Fluid
Chanon Suntara,
Metha Wanapat,
Sompong Chankaew,
Benjamad Khonkhaeng,
Chanadol Supapong,
Pin Chanjula,
Pongsatorn Gunun,
Nirawan Gunun,
Suban Foiklang,
Kampanat Phesatcha,
Anusorn Cherdthong
Affiliations
Chanon Suntara
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Metha Wanapat
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Sompong Chankaew
Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Benjamad Khonkhaeng
Department of Agricultural Technology and Environment, Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000, Thailand
Chanadol Supapong
Department of Animal Science, Faculty of Agriculture, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand
Pin Chanjula
Animal Production Innovation and Management Division, Faculty of Natural Resources, Hat Yai Campus, Prince of Songkla University, Songkhla 90112, Thailand
Pongsatorn Gunun
Department of Animal Science, Faculty of Natural Resources, Sakon Nakhon Campus, Rajamangala University of Technology-Isan, Phangkhon 47160, Thailand
Nirawan Gunun
Program in Animal Production Technology, Faculty of Technology, Udon Thani Rajabhat University, Udon Thani 41000, Thailand
Suban Foiklang
Faculty of Animal Science and Technology, Maejo University, Chiangmai 50290, Thailand
Kampanat Phesatcha
Department of Animal Science, Faculty of Agriculture and Technology, Nakhon Phanom University, Nakhon Phanom 48000, Thailand
Anusorn Cherdthong
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
The purpose of this study was to determine how ruminal Crabtree-negative yeast affects the nutritional characteristics of winged bean (Psophocarpus tetragonolobus) tubers (WBT), in vitro gas and digestibility, and rumen fermentation. The experiment was carried out in a randomized complete design with a 5 × 2 (+1) factorial arrangement. Factor A determined the WBT products (a1 = dry WBT, a2 = fermented WBT without yeast in media solution, a3 = fermented WBT with Pichia kudriavzevii KKU20, a4 = fermented WBT with Candida tropicalis KKU20, and a5 = fermented WBT with Saccharomyces cerevisiae), whereas factor B determined the level of fermented WBT replacing cassava chips (b1 = WBT at 50% and b2 = 100% levels). The results of the experiment showed that the fermentation approach could increase the crude protein (CP) content of WBT by around 7% (p p P. kudriazevii KKU20 yeast strain had a 17.3% higher final asymptotic gas volume (Vf) than the C. tropicalis KKU20. Crabtree-negative yeast had a higher in vitro dry matter digestibility (IVDMD) than Crabtree-positive yeast after 12 h of incubation (p p C. tropicalis KKU20 enhanced propionic acid (C3) concentrations when cassava chips were replaced for half of all of the diet (C3 ranged from 26.0 to 26.4 mol/100 mol; p p < 0.01). According to our findings, nutritional enrichment with yeast might increase the in vitro gas production and digestibility of WBT. The study also demonstrated that Crabtree-negative yeast has a promising lead in terms of improving rumen fermentation quality. However, further research is required before deciding on an effective approach for optimizing the potential of WBT as a feed source.
