Exploring the anti-invasive effects of Ganoderma tsugae (Songshan Lingzhi) against metastatic melanoma: Insights from an in vivo study on oxidative stress modulation

Yuan-Hong Lan; Po Chun Lee; Yun-Sheng Lu; Evelyn Adela Nathania; Chia-Hung Kuo; Logan Takemoto; Hui-Min David Wang

Journal of Functional Foods (May 2024)

Exploring the anti-invasive effects of Ganoderma tsugae (Songshan Lingzhi) against metastatic melanoma: Insights from an in vivo study on oxidative stress modulation

Yuan-Hong Lan,
Po Chun Lee,
Yun-Sheng Lu,
Evelyn Adela Nathania,
Chia-Hung Kuo,
Logan Takemoto,
Hui-Min David Wang

Affiliations

Yuan-Hong Lan: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan
Po Chun Lee: Cardiovascular Clinic, Kaohsiung Armed Forces General Hospital, Kaohsiung City 802, Taiwan
Yun-Sheng Lu: Taiwan Agriculture Research Institute, Council of Agriculture, Taichung, Taiwan
Evelyn Adela Nathania: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan; Department of Nutritional Sciences, College of Agriculture, Health and Natural Resources, University of Connecticut, Storrs, CT 06269, United States
Chia-Hung Kuo: Department of Seafood Science, National Kaohsiung University of Science, Kaohsiung 811, Taiwan
Logan Takemoto: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan
Hui-Min David Wang: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 701, Taiwan, ROC; Corresponding author.

Journal volume & issue: Vol. 116
p. 106169

Abstract

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Ganoderma tsugae, a medicinal mushroom and one of Taiwan's significant commercially cultivated species, contains polysaccharides and triterpenoids reported to possess anticancer effects. This study investigated the G. tsugae water extract (GTWE) for its inhibitory mechanism against lung metastatic melanoma (LMM) and B16F10 cells. The antioxidant capacity and flow cytometry showed that GTWE reduced the production of cellular reactive oxygen species dose-dependently. MTT and wound healing assays further demonstrated that GTWE suppressed melanoma cell migration and viability. qRT-PCR and western blotting verified mechanisms of apoptosis, GTWE decreased the BCL2 expressions and upregulated Bax and cleaved caspase-9 protein. These findings suggest GTWE is a potential therapeutic agent in vitro and in vivo for treating melanoma cancer.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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