Antifungal activity of vanilla juice and vanillin against Alternaria alternata

Abstract

Vanilla juice has been shown empirically to have antifungal activity against some fungal strains; however, there are no activity reported against Alternaria genre. In this work, the chemical profile of vanilla juice was obtained and its antifungal activity against fungal strains from the family Pleosporaceae, isolated from sorghum- and barley-diseased plants, was tested. The strains were identified as Alternaria alternata by their molecular and morphological characteristics. The vanilla juice characterization from Vanilla planifolia pods showed the presence of vanillin, vanillic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, guaiacol, glucovanillin, vanillyl alcohol, and furfural. Vanilla juice showed a fungistatic effect against all A. alternata strains tested in this study and increased the lag time from 50 to 112 h, and no conidia were produced. This result indicates the possible application of vanilla juice as an alternative to control agricultural crops such as barley and sorghum in Mexico.

Keywords

• alternaria alternata
• antifungal activity
• fungistatic effect
• plant pathogens
• vanillin