Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9)

Yunjiao Chen; Junjiang Wang; Yangwen Ou; Hongzhang Chen; Suyao Xiao; Guo Liu; Yong Cao; Qingrong Huang

Journal of Functional Foods (Mar 2014)

Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9)

Yunjiao Chen,
Junjiang Wang,
Yangwen Ou,
Hongzhang Chen,
Suyao Xiao,
Guo Liu,
Yong Cao,
Qingrong Huang

Affiliations

Yunjiao Chen: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA
Junjiang Wang: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China
Yangwen Ou: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China
Hongzhang Chen: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China
Suyao Xiao: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China
Guo Liu: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China
Yong Cao: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; Corresponding authors. Tel./fax: +86 20 85286234 (Y. Cao). Tel.: +1 848 932 5514; fax: +1 732 932 6776 (Q. Huang).
Qingrong Huang: Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA; Corresponding authors. Tel./fax: +86 20 85286234 (Y. Cao). Tel.: +1 848 932 5514; fax: +1 732 932 6776 (Q. Huang).

Journal volume & issue: Vol. 7
pp. 737 – 745

Abstract

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An activity-guided isolation process was performed to identify the antioxidant constituents of Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9). The ethyl acetate extract showed the highest activity in scavenging DPPH radical among five different fractions extracted from 70% aqueous ethanol extract of Eucalyptus leaves. Nine known compounds possessing radical scavenging activity, which included gallic acid (1), quercetin-3-O-β-d-glucuronide (Q-3-G-A) (2), ethyl gallate (3), tellimagrandin II(4), chlorogenic acid (5), 3-O-galloyl-4,6-O-[(S)-hexahydroxydiphenoyl]-d-glucose (gemin D) (6), pedunculagin (7), tellimagrandin I (8), and 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG) (9) were isolated and identified from the ethyl acetate extract. Our results indicated that ethyl acetate extract and hydrolysable tannins among nine compounds exhibited great antioxidant activity in both chemical-based (e.g. DPPH and ABTS assays) and cellular-based (i.e., Hep G2) antioxidant assays, and PGG showed the highest cellular antioxidant activity. This study suggested that ethyl acetate extract and the hydrolysable tannins in Eucalyptus leaves would be promising natural antioxidants in pharmaceutical, feed and food supplement industries.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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