Development of a real-time LAMP assay for monofloral honey authentication using rape honey

Abstract

The objective of this study was to establish a real-time LAMP assay for authentication of rape (Brassica napus) honey to protect consumers from commercial honey adulteration. The LAMP primers targeting the internal transcribed spacer (ITS) of Brassica napus were designed, and its specificity was tested. The LAMP reaction temperature was also optimized, and the detection limit of the LAMP assay was determined with a serial dilution of genomic DNA from the seeds of Brassica napus. The results showed that the real-time LAMP assay can accurately and specifically detect the rape component in honey, and the detection limit was 10 pg genomic DNA of Brassica napus. Data on monofloral honey samples indicate that the real-time LAMP assay was 100% in concordance with the reported TaqManTM PCR assay. This study provides a promising solution for facilitating the authentication of rape honey in food retail market.

Keywords

• loop-mediated isothermal amplification (lamp)
• rape honey
• internal transcribed spacer (its)