Antibiogram Study of Bacterial Pathogen from Tilapia Fish in Bangladesh

Abstract

Bacterial pathogens are isolated, identified and antibiogram were performed by taking the skin, gills and intestine of twenty randomly selected Tilapia fish (Oreochromis niloticus) that collected from local market of the Dinajpur city, Bangladesh. A serial dilution was prepared with the stated sample and at the amount of 0.1 ml was plated on nutrient agar, differential also specific media respectively. Then gram’s staining, colony morphology, biochemical test and antibiogram performed respectively. The four different isolated species with frequency of occurrence are 31(40.26%) Escherichia coli, 3 (29.87%) Staphylococcus spp., 13 (16.88%) Pseudomonas spp., 10 (12.99%) Salmonella spp. respectively. Some of these pathogens have tendency to transmit to man, who eat fish or deal with fish and fish products. Amoxicillin, Cefixime, Azithromycin, Chloramphenicol, Ciprofloxacin, Penicillin G, Erythromycin, Vancomycin, Gentamicin and Neomycin antibiotics was performed during sensitivity test. Among the total (77) isolated bacteria were sensitive to gentamycin, chloramphenicol, ciprofloxacin and Azithromycin but resistant to Amoxicillin, Penicillin G, Vancomycin and Erythromycin. The study was conducted in term of medical importance. Hence it is considered that a variety of bacterial species can be associated with fresh Tilapia fish related pathogen to humans.

Keywords

• bacteria, fish sample, antibiogram and oreochromis niloticus.