An ACE inhibitory peptide from Isochrysis zhanjiangensis exhibits antihypertensive effect via anti-inflammation and anti-apoptosis in HUVEC and hypertensive rats

Yu Pei; Shengxuan Cai; Bomi Ryu; Chunxia Zhou; Pengzhi Hong; Zhong-Ji Qian

Journal of Functional Foods (May 2022)

An ACE inhibitory peptide from Isochrysis zhanjiangensis exhibits antihypertensive effect via anti-inflammation and anti-apoptosis in HUVEC and hypertensive rats

Yu Pei,
Shengxuan Cai,
Bomi Ryu,
Chunxia Zhou,
Pengzhi Hong,
Zhong-Ji Qian

Affiliations

Yu Pei: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China
Shengxuan Cai: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China
Bomi Ryu: Department of Marine Life Sciences, Jeju National University, Republic of Korea; Corresponding authors at: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China (Z.J. Qian).
Chunxia Zhou: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China
Pengzhi Hong: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China
Zhong-Ji Qian: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China; Corresponding authors at: College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Zhanjiang 524088, Shenzhen 518114, China (Z.J. Qian).

Journal volume & issue: Vol. 92
p. 105061

Abstract

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Microalgae are primary producers in marine ecosystems, and have been diffusely used in medicine and food. In this research, we evaluated the protective effects of polypeptides from microalgae, Isochrysis zhanjiangensis (EMFGTSSET, ETT) on endothelial injury in angiotensin II (Ang II)-induced human umbilical vein endothelial cells (HUVEC). The results showed that ETT inhibited the fluorescence intensity of intracellular reactive oxygen species (ROS), and reduces the expression of related cytokines, such as IL-1β, IL-8, TNF-α, iNOS, COX-2, ET-1, AT-1, and adhesion factor. It blocks inflammation and apoptosis through mitogen-activated protein kinases (MAPK), nuclear factor κB (NF-κB) and serine/threonine kinase (Akt) signal pathways, thereby alleviating endothelial damage. Meanwhile, ETT (IC50 = 15.08 μM) antagonizes Angiotensin-I Converting Enzyme (ACE) in a non-competitive binding mode, and has an antihypertensive effect in spontaneously hypertensive rats (SHR). Therefore, ETT has excellent effect in regulating hypertension, can become the main component of potential functional food for the prevention of cardiovascular diseases, and further improve the use value of Isochrysis Zhanjiangensis.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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