Establishment of an Indirect Competitive ELISA for Zearalenone

Abstract

In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action time of 15 min were determined the optimal conditions. The results showed that the detection range (IC20~IC80) was 11~292 pg/mL, and the minimum detection limit (IC10) was 6 pg/mL. The recoveries of the zearalenone from corn flour were 81.29%~105.80%. There was no cross reaction with ochratoxin A, aflatoxin B1, deoxynivalenol, fumonisin B1, T-2 toxin. The ic-ELISA could be used for detection of zearalenon in corn flour.

Keywords

• zearalenone
• toxin
• detection
• indirect competitive enzyme linked immunosorbent assay (ic-elisa)