Jurnal Teknologi dan Industri Pangan (Jun 2018)

AMYLASE INHIBITION AND FREE RADICAL SCAVENGING ACTIVITIES OF WHITE TURMERIC EXTRACT AND FRACTIONS

  • Dwiyati Pujimulyani,
  • Wisnu Adi Yulianto,
  • Astuti Setyowati,
  • Seila Arumwardana,
  • Annisa Amalia,
  • Hanna Sari W. Kusuma,
  • Ervi Afifah

DOI
https://doi.org/10.6066/jtip.2018.29.1.10
Journal volume & issue
Vol. 29, no. 1
pp. 10 – 18

Abstract

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Diabetes is the most common endocrinal disorder characterized by hyperglycemia and long-term complications. Recently, the development of antidiabetic drugs have focused on natural products with va-rious mechanisms such as the inhibition of α-amylase. White turmeric (Curcuma mangga Val) from Zinge-beraceae family has been reported to have antidiabetic activities, thus the aim of this study was to eva-luate the effects of C. mangga extracts and fractions as antioxidant and antidiabetic agents through sca-venging activities and inhibition of α-amylase. In this study, the antidiabetic activities of four fractions of C. mangga extracts (water, hexane, ethyl acetate, butanol), a C. mangga extract and butylated hydroxyto-luene/antioxidant standard were measured using α-amylase activity assay, while the antioxidant activities of the fractions were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylben-zothiazoline-6-sulfonic acid) (ABTS) assays. These fractions were also compared to an antidiabetic drug, acarbose, as a control and butylated hydroxytoluene (BHT), a synthetic antioxidant. For the antioxidant assay, the butanol fraction of C. mangga (BCM) showed the highest ABTS-reducing activity (IC50= 24.23 ±2.77 μg/mL), while with the DPPH assay, the ethyl acetate fraction (EACM) had the highest activity (IC50 = 83.95±2.89 μg/mL) as compared to the other fractions and C. mangga extract, but the activities were lower than that of BHT. For the antidiabetic assay, C. mangga extract (CME) had the highest α-amylase inhibitory activity (IC50 = 363.67 µg/mL) among other fractions, although lower than acarbose. Curcuma mangga fractions (BCM and EACM) had antioxidant activities, while C. mangga extract (CME) had a po-tential as an antidiabetic by in vitro studies. Further in vivo studies is needed to confirm these findings.

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