Comparison of Different Cell-wall Disruption and Fatty Acid Extraction from Dunaliella Salina Microalgae

Abstract

As extracted oil from microalgae is highly affected by selected cell-wall breaking method and type of the solvent used, thus an appropriate choice matters in which it might affect the quantity. This study was conducted to determine the most effective method on Dunaliella salina microalga cell disruption and solvent by comparing several methods. According to the results, the most efficient technique for oil extraction from Dunaliella salina microalgae was recorded as combination of enzymatic and homogenization methods (2.26±0.02 g.L-1), followed by enzymatic method with 3% cellulose and 1.5% flavourzyme (2.04±0.02 g.L-1), and finally ultrasonication (1.61±0.00 g.L-1). Based on the fatty acid profile, C16:0, C18:1 and C18:2 fatty acids were recorded as the main constituents ethanol was the most effective solvent by extraction of 8.22%, 1.07% and 5.18% of above mentioned fatty acids. Furthermore, present results demonstrated that in order to efficiently extract lipid from Dunaliella salina, enzymatic and homogenization methods exhibited the most efficient technique for cell disruption.

Keywords

• cell destruction
• dunaliella salina
• homogenization
• lipid extraction