Anti-inflammatory and anti-proliferative activities of natural and sulphonated polysaccharides from Pleurotus eryngii

Abstract

Polysaccharides were extracted from Pleurotus eryngii (PEPS) and sulphonated. Anti-inflammatory activities of PEPS and sulphonated PEPS (S.PEPS) were tested on RAW264.7 macrophages in four experimental models. Cytokines of IL-1β, IL-6, IL-10, and TNF-α were quantified by ELISA. Cytokine secretion ratios (IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10) were significantly decreased by PEPS and S.PEPS treatments in a dose dependent manner in all four experimental models. Anti-inflammatory activity of S.PEPS was significantly stronger than that of PEPS, and the S.PEPS exhibited stronger reactive oxygen species (ROS) and nitric oxide (NO) inhibition activities compared with those of PEPS. Additionally, S.PEPS also had significantly stronger anti-proliferative activities on both Caco-2 and HepG2 cells compared with those of PEPS. Results indicated that sulphonation improved the anti-inflammatory and anti-proliferative activities of PEPS significantly.

Keywords

• Pleurotus eryngii
• Polysaccharides
• Sulphonation
• Anti-inflammatory activity
• Anti-proliferative activity