Untargeted fecal metabolomics revealed biochemical mechanisms of the blood lipid-lowering effect of koumiss treatment in patients with hyperlipidemia

Bohai Li; Fan Hui; Zhang Yuan; Qingxiang Shang; Guo Shuai; Yingshu Bao; Yongfu Chen

Journal of Functional Foods (Mar 2021)

Untargeted fecal metabolomics revealed biochemical mechanisms of the blood lipid-lowering effect of koumiss treatment in patients with hyperlipidemia

Bohai Li,
Fan Hui,
Zhang Yuan,
Qingxiang Shang,
Guo Shuai,
Yingshu Bao,
Yongfu Chen

Affiliations

Bohai Li: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China
Fan Hui: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China
Zhang Yuan: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China
Qingxiang Shang: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China
Guo Shuai: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China
Yingshu Bao: Inner Mongolia Xilin Gol League Mongolian Hospital, Nadam West Street, Xilin Gol League 026000, China
Yongfu Chen: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, Inner Mongolia 010018, China; Corresponding author at: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, China.

Journal volume & issue: Vol. 78
p. 104355

Abstract

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Our previous study found that consuming koumiss daily could beneficially reduce the levels of blood lipids, but the exact mechanism is not clear. Thus, this work aimed to investigate the biochemical mechanism of lipid-lowering effect using untargeted metabolomics by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). The fecal metabolomes of hyperlipidemia patients who consumed koumiss daily were analyzed before and after koumiss treatment. A total of ten significant differential metabolites (ursolic acid, linoleic acid, stearic acid, α-tocotrienol, γ-tocotrienol, alanine, tyrosine, sphingosine, acetate, and butyrate) were detected, and they all showed significant increased relative concentrations at days 30 and 60. Many of these metabolites were involved in lipid metabolism and transport. These results suggested that the ten identified metabolites were likely associated with the observed lipid-lowering effect. Finally, by using the Search Tool for Interactions of Chemicals (STITCH), metabolic networks connecting these metabolites were constructed to elucidate the biochemical mechanism of the hypolipidemic effect.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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