L-theanine relieves acute alcoholic liver injury by regulating the TNF-α/NF-κB signaling pathway in C57BL/6J mice

Abstract

Acute alcoholic liver injury (AALI) refers to the inflammatory hepatic damage caused by alcohol. In severe cases, it can lead to steatohepatitis, alcoholic fibrosis and even cirrhosis. In this study, we analyzed the hepatoprotective activity of L-theanine on mice fed with a large amount of ethanol at one time, and the underlying molecular mechanism. The extensive liver injury caused by alcohol feeding was alleviated by L-theanine, which reduced tissue damage, decreased transaminase levels, and increased antioxidant activity. Mechanistically, L-theanine downregulated phosphorylated NF-κB, TNF-α and IKKα in the liver tissues and the LO2 normal hepatocyte cell line, which in turn lowered secretion of iNOS, IL-1β and IL-6. Taken together, L-theanine prevents alcohol-induced liver inflammation by blocking the intrahepatic TNF-α/NF-κB pathway, and is a promising treatment strategy against AALI.

Keywords

• L-theanine
• Acute alcoholic liver injury
• Anti-inflammation
• TNF-α
• NF-κB pathway