Effects of Lipopolysaccharide as an Inducer on the Mitochondrial Apoptosis Signaling Pathway in Tan Sheep Muscle Cells during Postmortem Aging

Abstract

In order to investigate the effects of lipopolysaccharide (LPS) on the mitochondrial signaling pathway in muscle cells of Tan sheep, M longissimus dorsi muscles from six-month-old Tan sheep were aged at 4 ℃ for 0, 6, 12, 24 and 72 h postmortem and evaluated for pH, ATP content, cell apoptosis rate, cell apoptosis-related enzyme activity, succinatedehydrogenase (SDH) activity, citrate synthase (CS) activity, B-cell lymphoma 2 (Bcl-2) family protein contents, and cytochrome c (Cyt-c) and cysteinyl aspartate specific proteinase 3 (Caspase-3) expression levels. The results showed that the apoptosis rate and Bcl-2-associated X protein (Bax)/Bcl-2 ratio in the control and LPS-induced groups were significantly increased during postmortem aging (P < 0.05). The activities of Caspase-3 and Caspase-9 increased first and then decreased. After LPS treatment, the activities of Caspase-3 and Caspase-9 were higher than those in the control group at all time points. The pH, Cyt-c reduction level and mitochondrial ATP content were significantly decreased overall (P < 0.05). The relative expression level of Cyt-c was significantly increased in both groups at 12 h postmortem, and decreased during 24–72 h in the LPS group. The content of Caspase-3 activated fragment protein in the LPS treatment group was higher than that in control group at the same time points. In conclusion, LPS could activate Caspase-3 and Caspase-9, and reduce the mitochondrial content of ATP and consequently energy metabolism level, thus affecting the activities of SDH and CS, promoting the release of Cyt-C, and increasing Bax/Bcl-2 ratio, and finally inducing cell apoptosis through the mitochondrial signaling pathway.

Keywords

• tan sheep; lipopolysaccharide; cell apoptosis; mitochondrial signaling pathway